> Attachment to some resin based on charge or a binding site can be used in a chromatography setup. Solubility or inertness in some chemical can help. Finally regular filtration or some sort of hollow fibre tff setup.
Yep. A very typical workflow would be preliminary semi-purification over an affinity column followed by isolation of your protein of interest over a size exclusion column. Just one, but very common, method.
NosemaCeranae t1_irbbvy9 wrote
Reply to comment by Mikedc1 in is it possible to synthesize proteins chemically? by yeeturking
> Attachment to some resin based on charge or a binding site can be used in a chromatography setup. Solubility or inertness in some chemical can help. Finally regular filtration or some sort of hollow fibre tff setup.
Yep. A very typical workflow would be preliminary semi-purification over an affinity column followed by isolation of your protein of interest over a size exclusion column. Just one, but very common, method.