OneDayCloserToDeath t1_jdwtzoj wrote on March 27, 2023 at 7:45 PM

Reply to comment by JackD4wkins in Scientists discover how cancer cells evade immune system by BousWakebo

And how do you stop the virus from infecting healthy cells and causing more harm than good?

Toranagas1 t1_jebqx8n wrote on March 30, 2023 at 9:18 PM

The technology they is referring to uses whole genome sequencing to identify InDel mutations in tumor cells then uses Cas9/gRNA specifc for those InDel sequences to induce double stranded DNA breaks, resulting in cell death. Since those sequences don't in theory exist elsewhere in the patient, it may be safe. Looks like a lot of different gRNAs are needed though for good efficacy.

They use lentivirus and AAVs to deliver them in vivo.

The in vivo efficacy data is...fine. The big thing here is the personalized medicine aspect.

PMID: 35217600

JackD4wkins t1_jdxda7b wrote on March 27, 2023 at 9:50 PM

Because you coded the the virus enzyme to specifically target cancer DNA and not healthy DNA. Even if the virus infects a healthy cell, it will have no effect

OneDayCloserToDeath t1_jdxhmrz wrote on March 27, 2023 at 10:21 PM

Is this done? Do you have any information on it working?

JackD4wkins t1_jdxjblw wrote on March 27, 2023 at 10:33 PM

They're working on it in South Korea. Its called the CINDELA program. Not sure what the status is

ecnecn t1_jdzehcr wrote on March 28, 2023 at 9:08 AM

>CINDELA program

You mean Cancer-Specific InDel Attacker (Cindela) ? I didnt know they use reprogrammed viruses...