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Rtheguy t1_ira7mr0 wrote

Yes, it is however incredibly cumbersome and difficult to get to any size and the folding is sometimes aided by specific cell organels. So if you have a protein you want, just make it in e.coli. As for the DNA part, we can design DNA de novo aswell. It is also cumbersome and slow, but you can print a bit of DNA. So if you want to make an engineered enzyme just do that, it is for now much easier.

Folding of proteins and post transcription modifications are significant enough and different that for instance insulin can only be made in eukaryotes if you want it to really function well in human systems.

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CrateDane t1_irb9tcb wrote

> > > > > Folding of proteins and post transcription modifications are significant enough and different that for instance insulin can only be made in eukaryotes if you want it to really function well in human systems.

Well, there are ways around it. Some companies produce insulin in bacteria, while others produce it in yeast. It's a tradeoff since yeast is better at making it correctly in the first place, but is otherwise a less efficient and more complicated expression system than bacteria.

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