Submitted by AutomaticAd1918 t3_z67gnl in askscience
Plantpong t1_iy0b0x7 wrote
Reply to comment by FogeltheVogel in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
Agreed with the above! NHEJ is often used for the insertion of 'random' nucleotides for generating gene knockouts. HR can more efficiently be used for targeted insertions when also introducing a repair template along with the CRISPR complex that has homology arms that match the target splice site.
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