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-Metacelsus- t1_iy19ucd wrote

Reply to comment by Astavri in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918

Doing it with the cas9 plasmid is cheaper and works nearly as well. There is a greater chance of off-target edits though. But yes, if I was doing it clinically I would use the ribonucleoprotein.

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Astavri t1_iy1ba6r wrote

That's clever. I never thought about that as an option.

Do you have any publications or sources for doing it this way? If it's not any trouble.

I don't use crispr for anything at the moment, but there might be something I want to try it on. This 100% seems like the better option. And you could reuse the same plasmid with the cas9 and guide correct me if im wrong, and change the genes on the second plasmid if you want to try introducing a different gene in the same region?

Is it Ecoli or mammalian cells you are editing?

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