Submitted by AutomaticAd1918 t3_z67gnl in askscience
CrateDane t1_iy4zwth wrote
Reply to comment by Astavri in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
You have multiple options. You can provide the Cas9 gene in DNA form, but also as mRNA. Or simply the protein, usually pre-assembled with guide RNA into a RNP.
Even the Cas9 DNA delivery has multiple options - viral vector, plasmid transfection, it all depends on the use case.
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