Viewing a single comment thread. View all comments

Bitchwar t1_iz42itz wrote

Reply to comment by tanteidaiko in What are Serotypes? Are they a kind of Phenotype / Genotype? by tanteidaiko

Not familiar with the lingo. Blast, is that a method in itself?

Maybe I can ask a couple of questions if you have the time: Are you sequencing serotype, transcriptomics and DNA of white blood cells that are infected? And can you predict serotype from DNA? Or only from transcriptomics?

1

tanteidaiko OP t1_iz42y1y wrote

BLAST is an online sequence alignment tool by NCBI.

I am sequencing the genome of the Dengue virus itself that we extract from an infected person. And we are able to determine the serotype affecting a person via real time PCR itself. So we draw the patient's blood, spin it down into serums, then we do nucleic acid extraction to obtain the RNA. Then we do real time RT-PCR to detect the presence of any sereotype of the virus.

2