Stewy_434
Stewy_434 t1_irf2ll6 wrote
Reply to comment by smurfettekcmo in A bold effort to cure HIV—using Crispr by Sariel007
Restriction enzymes, or restriction endonucleases, or base pair cutters, cut doubled-stranded DNA by disrupting the phosphodiester bond that joins adjacent nucleotides. It is not random though.
Like other enzymes, restriction enzymes show specificity for certain substrates. For these enzymes, the substrate is a sequence of base pairs in the DNA strands. They bind to, recognize, and cut (digest) DNA within specific sequences, called restriction sites, and these sites must be palindromic readings from the separate strands. You can have 4, 6, or 8 (there are others) base pair cutters. If the sequence is shorter (say a 4 base pair cutter), it is more likely to be repeated than an 8-base pair cutter.
The biochemistry behind it is ridiculous and goes into why enzymes cut at the free ends of DNA molecules, while restriction enzymes cut inside the DNA strands.
Stewy_434 t1_itbpnj3 wrote
Reply to Tom Brady apologizes for his 'very poor choice of words' when comparing playing football to a military deployment by Sanlear
Bruh. As a veteran, who fucking cares. No rational person is sitting here going, "What the FUCK did he just say?? How dare he!" It's only irrational, bored people.
Let's win some football games so we can stop hearing about dumb stuff. Reminds me of F1's "silly season".