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corknut1 t1_iy1setu wrote on November 28, 2022 at 2:20 AM

Reply to comment by New_Concert_4315 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918

The initial end is bonded to a "support" (glass beads called cpg are typical) and only cleaved from the support once the entire chain is grown. The cleaving process also caps this end.