Submitted by AutomaticAd1918 t3_z67gnl in askscience
InaMellophoneMood t1_iy1ymsr wrote
Reply to comment by theartificialkid in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
Why would you do that when you can just stitch together many chunks using modern assembly techniques? That way you can use synthesis in the cheap, high yield part of its curve, and plasmid replication/purification to yield large quantities of your sequence for even cheaper. Fussing with super long synthesis with the flaws of existing chemistries doesn't make sense when it's more time and labor intensive than assembling them from medium length synthesis.
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