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wanson t1_iy3hkaj wrote

Reply to comment by HotDadBod1255 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918

They can be. It depends on what you're doing. For me, I am just correcting SNPs in cell lines so 50-60 bp is enough and they're only a couple hundred dollars or so.

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HotDadBod1255 t1_iy3l7ih wrote

Right, totally different needs here. I use them for in-vivo gene editing, so purity and precision are paramount.

Like most things out there, when you need super high purity and quality, it's gonna cost you a lot. In this case oligos are already a pain to make so it's ridiculously expensive.

To give you an idea, around 2000mg of the oligo I use as guide RNA will cost in the $2M range.

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