wanson
wanson t1_iy2uc5j wrote
Reply to comment by Aescwicca in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
It’s just an oligo. We use them as primers all the time in PCR. You just decide what bases you want and order it from a company. Small 20 base pair primers cost about $3.
They’re made by a chemical process and shipped dried (lyophilized). We just dissolve them in water or buffer.
wanson t1_iy3hkaj wrote
Reply to comment by HotDadBod1255 in How exactly does CRISPR-CAS9 insert new genes? by AutomaticAd1918
They can be. It depends on what you're doing. For me, I am just correcting SNPs in cell lines so 50-60 bp is enough and they're only a couple hundred dollars or so.