smurfettekcmo

smurfettekcmo t1_irftg4g wrote

RE are very different tech than CRISPR. CRISPR is much more specific to a certain seq I believe. RE sites are pretty specific but you tend to have multiple of each depending on the length of sequence. I left the molecular lab before CRISPR but have used RE digestion a lot personally when I was still in the lab.

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smurfettekcmo t1_irejk2s wrote

I happy to learn this research is taking place. It was one of my thoughts first learning of CRISPR. Why can’t we just use it to chop up viruses like Pac-Man? This is step further cutting out the imbedded sequences. I just hope the region the target is very specific to HIV. I know they mention it’s a well conserved region of HIV but with our DNA containing so much viral DNA already could it make edits in unintended regions?

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